OUTLINE OF COURSE

Biochemistry 1 (MEDS 350) is a four-credit course intended to provide graduate students with an introduction to basic concepts that underlie both the theoretical and practical aspects of biochemistry. The practical importance of many concepts will be highlighted with clinical correlations. Furthermore, the course will provide an introduction to topics on the cutting-edge of biochemical research such as proteomics, structural genomics, and microRNAs. In 2005, the course will consist of 40 lectures divided into three sections:

• Nucleotides, replication, transcription, and translation (14 lectures)
• Amino acids, proteins, and enzymes (13 lectures)
• Metabolism, membranes, and membrane transport (13 lectures)

In addition, there are six scheduled discussion/tutorial sessions that will be used at the discretion of each of the lecturers for additional lecture material, question-and-answer tutorials, or general discussion on specific topics.

Note that most lecturers post either lecture notes or a copy of their PowerPoint slide presentation on this website prior to their lecture. You can access this material by clicking on the Lecture material link above. You can also download self-study tutorials and sample exam questions by clicking on the Tutorials link above. The login/password for accessing these files will be given out during the first lecture.

There will be a two-hour, closed-book exam at the end of each section (i.e., three exams in total). If you cannot attend on the scheduled exam date for any reason, a new exam date will be set by the lecturer in charge. Please note that the rescheduled exam could be a written or oral exam, at the discretion of the lecturer in charge.

The lecture and exam schedule for the Fall 2005 course is given below. You can also download a PDF version of the complete syllabus. Please note that all lectures will be held from 10.15 to 11.15 am on Monday, Tuesday, Wednesday, and Friday in Room EG052 on the ground floor of the Academic Research Building (ARB).

DECEMBER 21: FINAL EXAM RESULTS

• You can pick up your Section 3 exam paper and receive your final grade from Dr. King' office (L-3096) anytime after 1.45 pm on December 21.

NOVEMBER 28: ON-LINE COURSE SURVEY

• The on-line course survey will become available on Blackboard on December 6th. Could you please try to complete the survey by December 16th or before going on your Winter break. I have attached some tips for completing the survey. Please note that the survey results are anonymous. We value your feedback and take it very seriously. Thanks!

A NOTE ABOUT BIOCHEMISTRY II

• Biochemistry II (Cool techniques in Biochemistry) is a 3-credit hands-on course designed to familiarize students with a variety of modern biochemical instrumentation that might be applicable to their PhD research. For those interested in the course, you can download a short or long version of the syllabus.

EXAMS

The date, time, and location of the three sectional exams are as follows:

Exam 1: 2.30-4.30 pm, Wednesday 28th September, ARB Small Seminar Room (EG052)

Exam 2: 2.30-4.30 pm, Friday 28th October, ARB Small Seminar Room (EG052)

Exam 3: 2.30-4.30 pm, Friday 2nd December, ARB Small Seminar Room (EG052)

Please try to arrive at least 5 minutes before the scheduled start of the exam. You will be given an opportunity to read over the paper before the scheduled time begins. Please let Dr. King know as soon as possible if you have a scheduling conflict.

  August 29

  Outline of the course

  Dr. Henry Furneaux

  August 30

  DNA primary and secondary structure

  Dr. Shlomo Eisenberg

  August 31

  DNA tertiary structure

  Dr. Shlomo Eisenberg

  September 2

  Enzymes that regulate DNA structure

  Dr. Shlomo Eisenberg

  September 5

  Labor Day Holiday

  No class

  September 6

  DNA synthesis

  Dr. Shlomo Eisenberg

  September 7

  DNA replication I: DNA replication enzymes

  Dr. Shlomo Eisenberg

  September 9

  DNA replication II: Replication fork formation and dynamics

  Dr. Shlomo Eisenberg

  September 12

  DNA repair

  Dr. Shlomo Eisenberg

  September 13

  Discussion/Tutorial Session

  Dr. Shlomo Eisenberg

  September 14

  The central dogma

  Dr. Henry Furneaux

  September 16

  Regulation of transcription

  Dr. Henry Furneaux

  September 19

  Posttranscriptional events I

  Dr. Henry Furneaux

  September 20

  Posttranscriptional events II

  Dr. Henry Furneaux

  September 21

  Translation of mRNA: end of the dogma

  Dr. Henry Furneaux

  September 23

  Regulation of gene expression by microRNAs

  Dr. Henry Furneaux

  September 26

  Discussion/Tutorial Session

  Dr. Henry Furneaux

  September 27

  Study day for Exam 1

  No class

  September 28

  EXAM ON SECTION 1 (2 HOURS)

  34% OF GRADE

  September 30

  The amino acids

  Dr. Glenn King

  October 3

  Protein ionization and buffers

  Dr. Glenn King

  October 4

  Protein purification and sequencing

  Dr. Glenn King

  October 5

  Techniques for mass determination

  Dr. Glenn King

  October 7

  Protein secondary and tertiary structure

  Dr. Glenn King

  October 10

  Columbus Day Holiday

  No class

  October 11

  Protein structure determination

  Dr. Glenn King

  October 12

  Protein folding

  Dr. Jeff Hoch

  October 14

  Structural genomics

  Dr. Glenn King

  October 17

  Discussion/Tutorial Session

  Dr. Glenn King

  October 18

  Enzymes

  Dr. Henry Furneaux

  October 19

  Catalytic strategies

  Dr. Henry Furneaux

  October 21

  Regulatory strategies

  Dr. Henry Furneaux

  October 24

  Protein-protein interactions

  Dr. Henry Furneaux

  October 25

  Proteomics

  Dr. David Han

  October 26

  Discussion/Tutorial Session

  Dr. Henry Furneaux

  October 28

  EXAM ON SECTION 2 (2 HOURS)

  33% OF GRADE

  October 31

  Biological membranes I

  Dr. Betty Eipper

  November 1

  Biological membranes II

  Dr. Betty Eipper

  November 2

  Transport through membranes I

  Dr. Betty Eipper

  November 4

  Transport through membranes II

  Dr. Betty Eipper

  November 7

  Biochemical communications I

  Dr. Betty Eipper

  November 8

  Biochemical communications II

  Dr. Betty Eipper

  November 9

  Discussion/Tutorial Session

  Dr. Betty Eipper

  November 11

  Overview of metabolism

  Dr. Peter Setlow

  November 14

  Glucose metabolism I

  Dr. Peter Setlow

  November 15

  Glucose metabolism II

  Dr. Peter Setlow

  November 16

  Pyruvate and acetylCoA metabolism

  Dr. Peter Setlow

  November 18

  Glucose and fatty acid synthesis

  Dr. Peter Setlow

  November 21

  Thanksgiving Layover

  No class

  November 22

  Thanksgiving Layover

  No class

  November 23

  Thanksgiving Layover

  No class

  November 25

  Thanksgiving Layover

  No class

  November 28

  Discussion/Tutorial Session

  Dr. Peter Setlow

  November 29

  Bioenergetics I

  Dr. Glenn King

  November 30

  Bioenergetics II

  Dr. Glenn King

  December 2

  EXAM ON SECTION 3 (2 HOURS)

  33% OF GRADE